Mass spectrometric separation and determination of N1,N12-diacetylspermine in the urine of cancer patients.
نویسندگان
چکیده
An ionspray ionization mass-spectrometric method for the determination of N1,N12-diacetylspermine (Ac2Spm) was developed using 15N-labeled Ac2Spm as the internal standard. Concentrations of Ac2Spm in the urine obtained from 17 cancer patients measured by the present method correlated well with those measured by ELISA, showing the usefulness of the two methods.
منابع مشابه
Evaluation of urine N1,N12-Diacetylspermine as potential tumor marker for urinary bladder cancer.
BACKGROUND N1,N12-diacetylspermine, a diacetylpolyamine which was recently identified in urine, appeared to be a useful tumor marker for a number of cancers. No valid data on urine diacetylspermine concentration in patients with urinary bladder cancer exist. AIM Evaluation of urine N1,N12-diacetylspermine concentrations in individuals with urinary bladder cancer. METHODS Urine samples were ...
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We obtained monoclonal antibodies against N(1),N(12)-diacetylspermine (DiAcSpm) and N(1),N(8)-diacetylspermidine (DiAcSpd), and developed two systems of competitive ELISA that utilize the antibodies and a common enzyme-labeled antigen to measure these di-acetylpolyamines. Cross-reactions with N(1)-acetylspermidine in the assay of DiAcSpm and with N8-acetylspermidine in the assay of DiAcSpd were...
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ورودعنوان ژورنال:
- Biological & pharmaceutical bulletin
دوره 25 3 شماره
صفحات -
تاریخ انتشار 2002